| 摘要 |
<p>The present invention is directed to a method for quantitative PCR amplification of deoxyribonucleic acids (DNA) from a sample containing PCR inhibitors such as biological, clinical or environmental samples. In the method of the invention an inhibitor-tolerant DNA polymerase is used in a pre-amplification step to increase the copy number of DNA from these samples. In the pre-amplification step, the PCR reaction preferably comprises at least the same amount of effective PCR inhibitors as a reaction with 1% (v/v) human blood. The pre-amplified sample is subsequently diluted in order to dilute inhibitory substances remaining in the sample and thus rendering possible to use an aliquot of the diluted sample in quantitative PCR, which is very sensitive for these inhibitors.</p> |
