A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo . The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.
申请公布号
EP2267131(A1)
申请公布日期
2010.12.29
申请号
EP20100177654
申请日期
1998.12.21
申请人
CARNEGIE INSTITUTION OF WASHINGTON;THE UNIVERSITY OF MASSACHUSETTS
发明人
FIRE, ANDREW;XU, SIQUN;MONTGOMERY, MARY K.;KOSTAS, STEPHEN A.;TIMMONS, LISA;TABARA, HIROAKI;DRIVER, SAMUEL E.;MELLO, CRAG C.