发明名称 |
PROCESS FOR PRODUCTION OF PEPTIDE THIOESTER |
摘要 |
Provided is a novel process for chemically converting a peptide chain into a peptide thioester. The inventors paid attention to the cysteine residue of a peptide chain. As a result, the following has been found. When a -C(=X)-R1 group is introduced to the thiol group of the cysteine residue and then the resulting peptide is reacted with a compound having a leaving group represented by the formula: -NH-C(=Y)NHR3 in an organic solvent, the -NH-C(=Y)NHR3 group binds via addition reaction to the carboxyl group of the N-terminal-side peptide bond of the cysteine residue, whereby the peptide bond is cleaved and the C-terminal-side peptide fragment is cut off. Further, when the resulting peptide chain having the -NH-C(=Y)NHR3 group is reacted with a thiol in a buffer solution, a thiol exchange reaction occurs, namely, the thiol group of the thiol binds to the carbonyl carbon to which the -NH-C(=Y)NHR3 group has bound, whereby the -NH-C(=Y)NHR3 group is eliminated. Thus, the conversion into a peptide thioester is attained. |
申请公布号 |
WO2010150730(A1) |
申请公布日期 |
2010.12.29 |
申请号 |
WO2010JP60443 |
申请日期 |
2010.06.21 |
申请人 |
OTSUKA CHEMICAL CO., LTD.;KAJIHARA, YASUHIRO;OKAMOTO, RYO;SAKAMOTO, IZUMI;ISHII, KAZUYUKI |
发明人 |
KAJIHARA, YASUHIRO;OKAMOTO, RYO;SAKAMOTO, IZUMI;ISHII, KAZUYUKI |
分类号 |
C07K1/06;C07K7/06;C07K14/00;C12P21/02 |
主分类号 |
C07K1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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