Immunostimulation by chemically modified RNA

摘要

Immunostimulatory agent (A) that contains at least one RNA (I) that has at least one chemical modification, is new. An independent claim is also included for a vaccine containing (A) and at least one antigen (Ag). ACTIVITY : Cytostatic; Virucide; Antibacterial; Fungicide; Protozoacide; Anti-HIV. MECHANISM OF ACTION : Vaccine; immune stimulation. (I) have a strong activating effect on antigen-presenting cells and immune cells, resulting in increased release of cytokines. Murine dendritic cells (DC) were stimulated with 10 Microg/ml of: (a) DNA 1668; or (b) RNA 1668, as phosphorothioate, for 6 days, then concentrations of interleukin (IL)-6 and -12 measured, in ng/ml. For (a) concentrations were about 23 (IL-6) and 2 (IL-12) but for (a) they were 30 and 11, respectively. 5'-UCCAUGACGUUCCUGAUGCU (RNA 1668) 5'-TCCATGACGTTCCTGATGCT (DNA 1668).