Method for the fermentative production of proteins

摘要

Producing a heterologous protein comprising more than 70 amino acids by utilizing an Escherichia coli strain in a fermentation medium comprises: (1) fermenting an E. colistrain in a fermentation medium such that the E. coli secretes the heterologous protein into the fermentation medium; and (2) removing the protein from the fermentation medium. Producing a heterologous protein comprising more than 70 amino acids by utilizing an Escherichia coli strain in a fermentation medium comprises: (1) fermenting an E. colistrain in a fermentation medium such that the E. coli secretes the heterologous protein into the fermentation medium; and (2) removing the protein from the fermentation medium. The E. coli strain has a mutation in the lpp gene or in the promoter region of the lpp gene, and a gene coding for a heterologous protein which is functionally linked to a signal sequence coding for a signal peptide. Independent claims are: (1) a method of secreting a protein; consisting of several subunits; and (2) an isolated E. coli strain.