发明名称 |
Improved methods for extracting nucleic acids from paraffin-embedded tissues |
摘要 |
<p>The present invention provides rapid and highly effective methods for extracting nucleic acids suitable for PCR amplification from tissue samples and paraffin-embedded tissue samples. Extraction is accomplished within a few minutes using a composition comprising a buffer, at least one nonionic surfactant, and a protease enzyme. The sample is then heated at alkaline pH and after a centrifugation step, the DNA is the supernatant can be used directly in known amplification methods, such as PCR.</p> |
申请公布号 |
EP2267127(A1) |
申请公布日期 |
2010.12.29 |
申请号 |
EP20100075363 |
申请日期 |
1999.04.27 |
申请人 |
ORTHO-CLINICAL DIAGNOSTICS, INC. |
发明人 |
BELLY, ROBERT T.;CHILSON, GARY J. |
分类号 |
C12N15/00;C12N15/10;C12N9/50 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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