| 摘要 |
FIELD: medicine. ^ SUBSTANCE: invention relates to medicine, in particular to physiology, biochemistry and can be used for express diagnostics of organism adaptation to hypokinesia both in usual conditions, and during space flights. Blood parametres are determined in patients only with hypokinesia and with hypokinesia at the background of desmopressin introduction, obtained results are compared and conclusion is made, as parametres mass-spectral determination of proteomic profiles of blood serum in mass range from 1000 to 17000 with processing of samples with magnetic particles MB WCX and MB IMAC Cu is carried out, and if there is no change in signal intensity after introduction of desmopressin with pre-fractioning by magnetic particles MB IMAC Cu m/z=9133 in comparison with initial data, as well as with reliable reduction of peak intensity m/z=1297, m/z=2769, m/z=7764, m/z=6432, m/z-6630 when pre-fractioning with magnetic particles MB WCX in these users stage of organism adaptation to hypokinesia with introduction of desmopressin is diagnosed. ^ EFFECT: method acceleration, possibility to determine about 20 parametres during one analysis, minimal amount of biomaterial (5-10 mcl), as well as possibility of said technique automation. ^ 5 cl, 3 tbl, 1 dwg |
