AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA, DNA FRAGMENT CODING AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA, AND METHOD OF OBTAINING AlpA LYTIC PROTEASE OF LYSOBACTER SP XLI BACTERIA

摘要

FIELD: chemistry. ^ SUBSTANCE: invention discloses AlpA lytic protease of Lysobacter sp. XL1 bacteria.The invention also relates to a DNA fragment of Lysobacter sp. XL1 bacteria which codes AlpA lytic protease. The invention also discloses a method of obtaining AlpA lytic protease through renaturation in vitro, involving cloning a section of a DNA fragment in claim 2 in Escherichia coli bacteria cells, where the said section contains an alpA gene fragment of Lysobacter sp.XL1 bacteria, which codes the proenzyme of AlpA lytic protease with or without violation of the signal peptide, production of this proenzyme in Escherichia coli bacteria cells, its aging in an inactive denatured state in form of inclusion bodies, renaturation and activation of the AlpA lytic protease in vitro. ^ EFFECT: invention widens the range of lytic proteases. ^ 3 cl, 4 dwg, 3 ex