| 摘要 |
FIELD: medicine. ^ SUBSTANCE: murine benign spontaneous breast tumour cells are cultivated. At first, the cultivation procedure is in vitro in the DMEM/RPMI-1640 medium with 10 % of bovine embryo serum up to 20 passages. Then, in vivo up to 22 passages. Then, once again in vitro up to 25 passages. Then, 10 thousand-1 million produced cells are introduced in a connective tissue capsule formed in the Balb/c mice after the introduction of a biocompatible gel. The tumour growth is enabled. The encapsulated tumour is aseptically removed and trypsinised to produce primary epithelial cell cultures. The produced cells concentrated 10 thousand-1 million are introduced into the capsule subcutaneously gel-formed in the Balb/c mice. The tumour growth is enabled for vaccination. In the in vitro culture, the cells are transplanted once a week while being disseminated in the ratio 1:3 - 1:4, with a monolayer twice washed with EDTA solution and removed with 0.25% trypsin during 2-3 min at room temperature, and the trypsinisation process is terminated by adding 10 % of fetal serum ".Ç.ü.". ^ EFFECT: invention allows producing an anticancer vaccine application of which is based on a principle of transplantation of a live pathological material to a person from an animal with similar disease. ^ 3 dwg |
