摘要 |
PROBLEM TO BE SOLVED: To adjust a condensing position of light in a more simple manner, when irradiating a sample with light of a plurality of different wavelengths. SOLUTION: An IR pulse laser 21 is configured to emit IR light for optically exciting the sample 24 by multiphoton excitation, the IR light passes through an illumination optical system 23 to irradiate the sample 24 therewith. A visible laser 22 is configured to emit visible light to develop a fluorescence from the sample 24, the visible light passes through an illumination optical system 25 to irradiate the sample 24 therewith. The IR light and the visible light have different wavelengths, so that the condensing position of the IR light is deviated from that of the visible light due to chromatic aberration of an objective lens 30, An angle of divergence of the IR light is however deflected by a beam expander 31 arranged in the illumination optical system 23, so that the condensing position of the IR light through the objective lens 30 is adjusted. The present invention is applicable to a scanning microscope. COPYRIGHT: (C)2011,JPO&INPIT |