发明名称 |
HIGH THROUGHPUT ASSAYS FOR THE PROTEOLYTIC ACTIVITIES OF CLOSTRIDIAL NEUROTOXINS |
摘要 |
In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can serve as immobilized substrates. In both types a fluorescent molecules is present in the substrate, eliminating the requirement for the addition of a fluorigenic reagent. The assays described can be readily adapted for use in automated or robotic systems.
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申请公布号 |
CA2451909(C) |
申请公布日期 |
2010.12.21 |
申请号 |
CA20012451909 |
申请日期 |
2001.09.25 |
申请人 |
U.S. ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES |
发明人 |
SCHMIDT, JAMES J.;STAFFORD, ROBERT G. |
分类号 |
C12Q1/37;C07K7/08;C07K14/33;C07K17/00;G01N33/542;G01N33/543;G01N33/569;G01N33/68 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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