| 摘要 |
FIELD: medicine, veterinary. ^ SUBSTANCE: invention relates to field of veterinary medicine. Method lies in the following: carried out is selection of affected organs of dead nutrias from local epizootic centre, from which suspension is prepared. After that performed is inoculation on differential-diagnostic media and pure cultures of causative agents of streptococcosis, pseudomonosis and enterococcal infection of nutria is carried out. After that, cultures of Streptococcus uberis, Pseudomonas aeruginosa and Enterococcus faecalis are separately grown in meat-peptone broth with addition of glucose to 0.2% concentration with titre 4-5 billion in 1 cm3, inactivated by introduction of formalin to 0.4-0.5% final concentration, kept at temperature 37C for 72-96 hours. After that, cultures are mixed in equal ratios, solution of aluminium hydroxide is introduced in amount 20% to the volume, thoroughly mixed and packed. ^ EFFECT: method allows to obtain safe, specific, highly immunogenic vaccine. ^ 1 tbl, 1 ex |
