| 摘要 |
The present invention is directed to a method with which one can detect a nucleic acid with sufficient accuracy, no matter the nucleotide length of a primer, and no matter if the analyte nucleotide consists of only a single nucleotide. Specifically disclosed is a method for detecting whether or not a nucleic acid sample contains a nucleic acid which has a target nucleotide sequence comprising one or more analyte nucleotide(s) in a nucleic acid sample. Firstly, in a step (a), a nucleic acid extension reaction is performed with use of a nucleic acid contained in the nucleic acid sample, an analyte nucleotide-identification primer, and a polymerase. Next, in a step (b), an extension product yielded from the step (a) is detected. The step (a) uses, as the primer, a polynucleotide which comprises a nucleotide sequence having an insertion site or a deletion site of one or more nucleotide(s), on the 5′ side of nucleotide(s) corresponding to the analyte nucleotide(s), in a nucleotide sequence homologous or complementary to an analyte nucleotide-containing partial region of the target nucleotide sequence.
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