| 摘要 |
PROBLEM TO BE SOLVED: To facilitate a comprehensive analysis of lipids, which is hardly separated by TLC, with a high throughput. SOLUTION: A plurality of lipids included in a sample are separated by TLC (S1) for transferring to a PVDF film from a TLC plate subjected to constituent separation (S2). The transferred PVDF film is stuck to a MALDI target plate by a conductive tape (S3), and a matrix is uniformly applied onto the surface (S4). A two-dimensional range on a sample thus prepared is measured by an imaging mass spectrograph to acquire mass spectra for each of a number of measurement points (S5). By creating a specific two-dimensional distribution image of m/z, based on the mass spectra, the distribution of lipids of molecular species having the same type and different structure that could not be separated by TLC can also be visualized. COPYRIGHT: (C)2011,JPO&INPIT |
