| 摘要 |
This invention relates, at least in part, to conditionally active ribozymes and uses of such ribozymes. Some aspects of this invention relate to the engineering of conditionally active ribozymes. In some embodiments, the splicing activity of such ribozymes is modulated by at least one regulatory element. Some aspects of this invention relate to uses of conditionally active ribozymes. RNA detection technology, conditional RNA expression technology, cell tagging technology, therapeutic approaches, and synthetic biology are examples of areas in which conditionally active ribozymes according to some aspects of the invention can be employed. RNA folding models useful in the design of conditionally active ribozymes with altered splicing efficiency and/or substrate specificity are provided. Compositions and methods to manufacture medicaments containing conditionally active ribozymes are also described.
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