SCREENING METHOD FOR THE IDENTIFICATION OF TNF-PATHWAY MODULATORS USING TRADD /REPORTER FUSION PROTEIN
摘要
The present invention relates inter alia to methods for identifying modulators of tumour necrosis factor (TNF) signalling. In one aspect, the method involves identifying an agent that modulates a signalling pathway mediated by TNF, comprising the steps of providing a host cell comprising TNF receptor 1 (TNFR1)-associated death domain (TRADD) linked to a reporter molecule, and determining at least one cellular characteristic detectable by the reporter molecule in the host cell in the presence of TNF and in the presence and absence of a candidate modulator. The invention is suitable for high-throughput screening (HTS) and high-content screening (HCS), or a combination of HTS and HCS.
申请公布号
WO2010082133(A3)
申请公布日期
2010.12.02
申请号
WO2010IB00142
申请日期
2010.01.19
申请人
ADDEX PHARMA S.A.;CHEDAL-BORNU GOUILLER, AURELIE, FLORENCE;MUTEL, VINCENT;LUET JENS, ROBERT, JOHANNES
发明人
CHEDAL-BORNU GOUILLER, AURELIE, FLORENCE;MUTEL, VINCENT;LUET JENS, ROBERT, JOHANNES