| 摘要 |
<p>The present invention is directed to a process of detecting a target nucleic acid using labelled oligonucleotides. This process uses a specific activity of a nucleic acid polymerase to cleave annealed labelled oligonucleotide from hybridized duplexes and release labelled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay, such as real-time or Taqman PCR, wherein the labelled probe overlaps with the primer that anneals on the same nucleic acid strand. Thus, a triplex is formed between target, probe and primer. A polymerase having nuclease activity is used, which cleaves the labelled (eg. with FRET) probe and releases labelled fragments.</p> |
