发明名称 |
SELECTION AND ENRICHMENT OF PROTEINS USING IN VITRO COMPARTMENTALIZATION |
摘要 |
<p>Compositions and methods are provided for selection and enrichment of a target gene from a library of polynucleotide sequences such as might be formed from a genome or by random mutagenesis of a genetic sequence. The selection and enrichment occurs in aqueous droplets formed in an emulsion that compartmentalize individual polynucleotides from the library or a plurality of polynucleotides that may include polynucleotides not derived from the library, transcription and translation reagents and optionally additional chemical and enzyme reagents. The selection and enrichment method utilizes a polynucleotide adaptor which when ligated to the polynucleotide fragment enables amplification to occur in the presence of an adaptor specific primer.</p> |
申请公布号 |
EP2118280(B1) |
申请公布日期 |
2010.12.01 |
申请号 |
EP20080730500 |
申请日期 |
2008.02.22 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
ZHENG, YU;ROBERTS, RICHARD, J. |
分类号 |
C12N15/10;C12N9/22;C12N15/55;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|