发明名称 |
METHODS FOR REFOLDING PROTEINS CONTAINING FREE CYSTEINE RESIDUES |
摘要 |
The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins. |
申请公布号 |
IL152804(A) |
申请公布日期 |
2010.11.30 |
申请号 |
IL20020152804 |
申请日期 |
2002.11.12 |
申请人 |
BOLDER BIOTECHNOLOGY, INC. |
发明人 |
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分类号 |
C12N15/09;C07K1/02;C07K1/113;C07K14/00;C07K14/505;C07K14/515;C07K14/52;C07K14/535;C07K14/56;C07K14/61;C07K14/78;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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