METHODS AND CULTURE SYSTEMS FOR PREPARING FEEDER CELLS - FREE, XENO - FREE HUMAN EMBRYONIC STEM CELLS AND STEM CELL CULTURES PREPARED USING SAME

摘要

A culture medium which comprises transforming growth factor ² 1 (TGF² 1 ) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, said culture medium being capable of maintaining pluripotent stem cell in an undifferentiated state.