发明名称 |
INHIBITOR OLIGONUCLEOTIDES AND THEIR USE FOR SPECIFIC REPRESSION OF A GENE ENCODING AN ANDROGEN RECEPTOR |
摘要 |
Double-stranded oligonucleotides (I) consisting of two complementary strands (Ia; Ib), each having at one of their 3' and 5' ends 1-5 unpaired nucleotides, forming single-stranded overhangs, are new. One of (Ia) and (Ib) is complementary to a target sequence, of DNA or RNA, that is to be specifically repressed. ACTIVITY : Cytostatic; Virucide; Anti-HIV; Neuroprotective; Ophthalmological; Antidiabetic; Antipsoriatic; Antirheumatic; Antiarthritic. The oligonucleotide hybrid (A) prepared from sequences of formulae ggggaggcagccauugagacTT gucucaauggcugccuccccTT (lower case indicates ribonucleotides and upper case deoxyribonucleotides) was designed to target the junction of the PML/RARalpha fusion gene associated with promyelocytic leukemia. It (500 ng) was introduced into NIH 3T3 cells, co-transfected with a vector (100 ng) that expressed the PML/RARalpha fusion. Western blotting analysis after 48 hours showed greatly reduced expression of the fusion protein, relative to a control without (A). MECHANISM OF ACTION : Antisense inhibition of gene expression. |
申请公布号 |
PT1453962(E) |
申请公布日期 |
2010.11.29 |
申请号 |
PT20020793219T |
申请日期 |
2002.11.08 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) |
发明人 |
ANNICK HAREL-BELLAN;SLIMANE AIT-SI-ALI;FLORENCE CABON-GEORGET;ANNE CHAUCHEREAU;FRANCOIS DAUTRY;LUIS MARTINEZ |
分类号 |
C12N15/09;C12N15/113;A61K31/7105;A61K31/711;A61K31/713;A61K38/00;A61K48/00;A61P9/10;A61P9/14;A61P17/06;A61P19/02;A61P25/00;A61P27/02;A61P29/00;A61P31/00;A61P31/12;A61P31/18;A61P35/00;A61P43/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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地址 |
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