Method for sorting sperm cells into X or Y chromosome-bearing enriched populations comprising a composition which inhibits motility and a DNA-selective dye

摘要

Disclosed is a method of making a gender enriched sperm cell suspension comprising: mixing a sperm cell suspension with a composition which inhibits motility to form a chemically immotile sperm cell suspension, staining said chemically immotile sperm cell suspension with a DNA-selective dye; and sorting the stained chemically immotile sperm cell suspension to form a viable, gender enriched insemination population of X chromosome bearing or Y chromosome bearing sperm cells having DNA-selected dye associated with their DNA. Alternatively the DNA-selective dye can be mixed with the sperm-cell suspension before the composition which inhibits motility has been added.