发明名称 COMPOSITION AND METHOD FOR USING SOLID SUPPORT TO PURIFY RNA
摘要 PROBLEM TO BE SOLVED: To provide a reagent, method and kit for purification of RNA from a biological sample. SOLUTION: There are provided a formulation for isolating and purifying nucleic acids comprising a lithium salt at concentration of at least about 1 M, a surfactant, and a buffer. The invention provides a formulation for isolating and purifying nucleic acids, in one embodiment, comprising at least a lithium salt at concentration of at least about 1 M, a surfactant, a buffer and if necessary a chelating agent, if necessary a reducing agent, and if necessary a tungstate, wherein the solution has a pH of above about 7. The invention further provides a method for purifying substantially pure and undegraded RNA from a biological samples containing RNAs. COPYRIGHT: (C)2011,JPO&INPIT
申请公布号 JP2010263919(A) 申请公布日期 2010.11.25
申请号 JP20100181955 申请日期 2010.08.16
申请人 QIAGEN NORTH AMERICAN HOLDINGS INC 发明人 BAIR ROBERT J JR;HEATH ELLEN M;MEEHAN HEATHER;PAULSEN KIM E;WAGES JOHN M JR
分类号 C12N15/09;C12N15/10;C12Q1/68 主分类号 C12N15/09
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