摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for specifically and quickly detecting and identifying the particularly virulent strain Listeria monocytogenes. <P>SOLUTION: Through classifying according to the single-nucleotide polymorphisms of hlyA gene, inlA gene, clpC gene and plcA gene, found in Listeria monocytogenes, and detecting a plurality of single-nucleotide polymorphisms, consisting of identified combinations classifiable according to the specific single-nucleotide permutations within the single-nucleotide polymorphism of each gene, the virulent strain belonging to the same group as the strain found in the clinical strain can be detected. In order to do this, PCR is applied, using, for example, a primer set comprising a primer having a specific base sequence and a primer having a specific base sequence. <P>COPYRIGHT: (C)2011,JPO&INPIT |