| 摘要 |
The inventors of the present invention have developed a novel cell culture method with a view to providing a process by which cell masses uniform in size and properties can be produced in large quantities and with convenience, as well as a method of recovering them. The inventors of the present invention found that the above-mentioned objects could be attained by using a structural member having a hollow portion at least one lower end of which is open, creating a projecting portion of a culture medium at the open lower end of the structural member, and culturing cells in that projecting portion of the culture medium. The present invention has been accomplished on the basis of this finding. Specifically, the present invention provides a cell culture method characterized by providing a structural member having a hollow portion at least one lower end of which is open, injecting a culture medium containing cells into the hollow portion of the structural member, causing a portion of the culture medium to project downward from the open end, and culturing the cells in the projecting portion of the culture medium. The present invention also provides a process for preparing cell masses characterized by forming cell masses by the above-described cell culture method.
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