摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method of making cDNA molecules and cDNA libraries. <P>SOLUTION: There are disclosed the method for synthesizing one or more cDNA molecules or a population of cDNA molecules, comprising: a process of mixing at least one mRNA or poly A RNA templates or a population of such templates with at least one polypeptide with reverse transcriptive activity; and a process of incubating the mixture under a condition enough to increase the amount or percentage of synthesized, full-length cDNA molecules, cDNA molecules and cDNA libraries produced according to these methods, vectors and host cells containing such cDNA molecules and libraries, and a kit for making the cDNA molecules and libraries. <P>COPYRIGHT: (C)2011,JPO&INPIT |