发明名称 cDNA SYNTHESIS IMPROVEMENT
摘要 <P>PROBLEM TO BE SOLVED: To provide a method of making cDNA molecules and cDNA libraries. <P>SOLUTION: There are disclosed the method for synthesizing one or more cDNA molecules or a population of cDNA molecules, comprising: a process of mixing at least one mRNA or poly A RNA templates or a population of such templates with at least one polypeptide with reverse transcriptive activity; and a process of incubating the mixture under a condition enough to increase the amount or percentage of synthesized, full-length cDNA molecules, cDNA molecules and cDNA libraries produced according to these methods, vectors and host cells containing such cDNA molecules and libraries, and a kit for making the cDNA molecules and libraries. <P>COPYRIGHT: (C)2011,JPO&INPIT
申请公布号 JP2010263905(A) 申请公布日期 2010.11.25
申请号 JP20100152752 申请日期 2010.07.05
申请人 LIFE TECHNOLOGIES CORP 发明人 LI WU BO;JESSEE JOEL A;GRUBER CHRISTIAN E
分类号 C12N15/09;C07K16/40;C07K16/44;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/99;C12N15/10;C12P21/08;C40B40/06 主分类号 C12N15/09
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