发明名称 |
Fusion Tag Comprising an Affinity Tag and an EF-Hand Motif Containing Polypeptide and Methods of Use Thereof |
摘要 |
The application discloses a fusion tag comprising an affinity tag and a polypeptide comprising one or more EF hand motif(s). Preferably, said fusion tag comprises a polyhistidine tag, one or more EF hand motif(s) of calmodulin and a thrombin cleavage site. Methods of using said fusion tag to purify a polypeptide of interest are also disclosed.
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申请公布号 |
US2010297734(A1) |
申请公布日期 |
2010.11.25 |
申请号 |
US20080682718 |
申请日期 |
2008.10.10 |
申请人 |
UNIVERSITY HEALTH NETWORK |
发明人 |
MCCLUSKEY ANDREW J.;POON GREGORY M.K.;GARIEPY JEAN |
分类号 |
C12N1/00;C07K1/22;C07K14/00;C12N15/00;C12N15/63 |
主分类号 |
C12N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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