摘要 |
The present invention provides a method for the extraction and isolation of solubilised arabinoxylan depolymerisation products, using arabinoxylan-containing plant material as a starting material. Preferably, such solubilised arabinoxylan depolymerisation product is any one of soluble arabinoxylan, arabinoxylan-oligosaccharides, xylose and arabinose. Said method comprises the mashing of said arabinoxylan-containing plant material in water and treating it with an appropriate concentration of an endoxylanase enzyme preparation in order to enzymatically depolymerise and solubilise a fraction of the arabinoxylans comprised in said plant material, wherein said endoxylanase treatment is followed or preceded by an acid treatment. This acid treatment comprising the incubation of said plant material in an acidified aqueous mash in order to obtain a depolymerisation and solubilisation of another fraction of the arabinoxylans comprised in said plant material. Preferably, said acidified mash is obtained by adding to said mash a strong acid to a final concentration between 0.02 and 0.4 N. The method further comprises the separation from said mashed plant material of a water-solubilised fraction, which comprises at least part of the solubilised arabinoxylan depolymerisation products. |