| 摘要 |
The present invention relates to processes for producing succinic acid and/or succinate ions by fermentation under anaerobic conditions which enable orotic acid production to be minimized. In particular, the invention provides a method for producing succinic acid, comprising: (a) a step of culturing a strain of ?scherichia coli in a culture medium, where said step (a) comprises: (a-1) a step of culturing the strain under aerobic conditions during which glucose is added to the culture medium according to a fed-batch mode, and during which the glucose concentration in said culture medium is less than 0.5 g/l; (a-2) optionally, an intermediate culture step; (b) a step of producing succinate ions by fermentation of the strain cultured in step (a), under anaerobic conditions in the presence of CO2; (c) a step of converting the succinate ions formed in step (b) into succinic acid; (d) optionally, a step of purifying the succinic acid. |
