发明名称 |
AMPLICON MELTING ANALYSIS WITH SATURATION DYES |
摘要 |
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
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申请公布号 |
US2010297656(A1) |
申请公布日期 |
2010.11.25 |
申请号 |
US20100833274 |
申请日期 |
2010.07.09 |
申请人 |
WITTWER CARL T;REED GUDRUN;DUJOLS VIRGINIE;ZHOU LUMING |
发明人 |
WITTWER CARL T.;REED GUDRUN;DUJOLS VIRGINIE;ZHOU LUMING |
分类号 |
C12Q1/68;C09B23/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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