| 摘要 |
FIELD: medicine. ^ SUBSTANCE: described is method of identification and intrageneric differentiation of strains of species Yersinia pestis, which includes separation of DNA from cell culture and carrying out testing with application of set of primers in PCR reaction, characterised in that testing is carried out in two stages: at the first stage for identification used is set of primers, consisting of primers Ç£3aÇ, Ç£vIml2for/ISrev216Ç, Ç£JSÇ, Ç£CDS39Ç, which interact with sample DNA, and synthesis of specific DNA, guided by primers Ç£3aÇ and Ç£vIml2for/ISrev216Ç confirms presence of plague causative agent in DNA sample, and positive result with primers Ç£CDS39Ç testifies to presence in DNA sample of Y. pestis strain of one of additional subspecies, intraspecific differentiation of which is performed at second stage by PCR with primers Ç£traAÇ, and Ç£YP02258Ç, obtained with each pair of primers results being estimated as positive if amplifiers of certain length are present, namely: Ç£3aÇ-276 pn, Ç£JSÇ - 223 pn, Ç£vIml2for/ISrev216Ç - 390 pn, Ç£traAÇ - 472 pn, Ç£CDS39Ç - 418 pn, Ç£YP02258Ç - 130 pn, results are considered negative if amplifiers are absent or present, but with uncharacteristic number of neucleotide pairs. ^ EFFECT: invention can be used in express-diagnostics of plague causative agent. ^ 7 cl, 11 tbl, 6 ex |
