FLUORESCENCE DETECTING METHOD AND FLUORESCENCE DETECTING DEVICE

摘要

Provided are a fluorescence detecting method and a fluorescence detecting device for calculating the fluorescence relaxation time constant more accurately than in the prior art when fluorescence emitted from an analyte is detected by applying laser beam. When the analyte passes the position irradiated with laser beam modulated in intensity by a modulation signal of a predetermined frequency, a first fluorescence signal of fluorescence received by a light-receiving means is collected. A second fluorescence signal of the fluorescence received by the light-receiving means is collected in a state in which the analyte is not in the position irradiated with the laser beam after the analyte has passed the position irradiated with the laser beam. Phase difference information of a fluorescence signal of the fluorescence emitted from the analyte with respect to the modulation signal of the laser beam is obtained by using the collected first fluorescence signal and second fluorescence signal, and the fluorescence relaxation time constant of the fluorescence emitted from the analyte is obtained from the obtained phase difference information of the fluorescence signal of the fluorescence emitted from the analyte.