发明名称 |
Compositions and methods of using a synthetic DNAse I |
摘要 |
<p>Compositions and methods for making and using a synthetic bovine DNase T are disclosed. More particularly, the sbDNaseI of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5'-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clcan-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling culture cells; tissue dissociation creation of fragmented DNA for in vitro recombination reactions.</p> |
申请公布号 |
EP2213741(A3) |
申请公布日期 |
2010.11.17 |
申请号 |
EP20100153371 |
申请日期 |
2004.03.31 |
申请人 |
AMBION, INC. |
发明人 |
LATHAM, GARY;KEMPPAINEN, JON |
分类号 |
C12N15/55;C07H21/04;C12N;C12N1/00;C12N9/22;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/55 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|