| 摘要 |
A method of producing a flagellin-based chimeric protein includes culturing a B. halodurans BhFD05 (&Dgr;hag, &Dgr;fliD, &Dgr;wprA, &Dgr;alp, &Dgr;apr, &Dgr;vpr, &Dgr;asp) strain deposited under Accession Number 41533 at the NCIMB. The strain is caused to express and secrete high levels of a flagellin-based chimeric protein into an extracellular growth medium. The flagellin-based chimeric protein comprises a heterologous peptide (i) inserted in-frame into a flagellin variable region which is flanked on its N-terminal side by an N-terminal fragment of a flagellin polypeptide and, optionally, flanked on its C-terminal side by a C-terminal fragment of a flagellin polypeptide, or (ii) fused to the C-terminal of a flagellin polypeptide.
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