摘要 |
Disclosed are methods of purifying an antibody-agent conjugate from unconjugated antibody and unconjugated agent. In some embodiments, the method comprises application of conditions to allow binding of a conjugate, an unconjugated antibody, and an unconjugated agent from a mixture of said components to a mixed mode chromatography support. The support is then contacted with a buffer such that the unconjugated antibody and unconjugated agent are substantially removed from the support while at least a majority of the conjugate remains bound to the support. Finally, the conjugate is eluted from the support, thereby purifying the antibody-agent conjugate from unconjugated antibody and unconjugated agent.
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