EXPRESSION CASSETTE FOR SECRETION/EXPRESSION OF POLYPEPTIDE, AND USE THEREOF

摘要

Disclosed are a method for the efficient secretion/expression of a polypeptide by means of a microorganism, preferably a bacterium belonging to the genus Bifidobacterium and a means for the method. Specifically disclosed is an expression cassette characterized by comprising: (i) a promoter selected from the group consisting of a promoter for a cbaH gene derived from a bacterium belonging to the genus Bifidobacterium, a promoter for rplJ gene derived from a bacterium belonging to the genus Bifidobacterium, and a promoter for hup gene derived from Bifidobacterium breve; (ii) DNA encoding a signal sequence selected from the group consisting of a signal sequence for a product of BL1181 gene derived from a bacterium belonging to the genus Bifidobacterium, and a signal sequence for a product of amyB gene derived from a bacterium belonging to the genus Bifidobacterium; and (iii) DNA encoding a polypeptide or a cloning site into which the DNA is to be inserted.