| 摘要 |
Apparatus and methods for freezing a tissue sample in preparation of sectioning. Generally, the apparatus comprises a chuck and a tissue heat sink that are adapted to rapidly freeze a tissue sample before being sectioned in a cryostat. While the chuck and heat sink may be made of any suitable material, in some cases, the chuck and heat sink include copper, a copper alloy, bronze, or a bronze alloy. To keep the chuck and heat sink from tarnishing, they can be covered with a non-tarnishing coating material, such as tetrafluoroethylene. Additionally, in order to secure the tissue sample that is embedded in a cutting medium to the chuck, the chuck preferably includes one or more dovetail-shaped apertures, such as a dovetail-shaped groove. |
