Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells

摘要

The present invention concerns a method of preparing a conditioned medium from a confluent stromal cell culture, the method comprising:
(a) culturing stromal cells to confluence in a stationary phase plug-flow bioreactor under continuous flow of medium on a three dimensional substrate to obtain a stationary phase three-dimensional confluent stromal cell culture, wherein said stromal cell culture is grown to a density of at least 5 x 10 6 cells per a cubic centimeter of said substrate; and
(b) separating and collecting a stromal cell conditioned medium from said stationary phase confluent stromal cell culture; thereby obtaining the conditioned medium.