发明名称 |
Method and device for platelets counting |
摘要 |
Counting thrombocytes (T) contained in a blood sample (E), comprises: mixing the sample with fluorescent markers to bind specifically with thrombocytes, and an agent for inhibiting the activation of the thrombocytes; introducing the sample in a fluid chamber (FC) having at least one transparent side; capturing at least one digital image (IN) of the sample by fluorescence microscopy under fixed lighting; and counting thrombocytes present in each image by computing means for treating images (O). An independent claim is included for an apparatus for counting thrombocytes, comprising: a fluid chamber in which a blood sample is introduced, comprising a wall at least partially transparent and containing an agent for inhibiting the activation of thrombocytes and fluorescent markers bind specifically with the thrombocytes; a light source for carrying out stationary lighting of the sample for exciting the fluorescent markers; an optical system of fluorescence microscopy for capturing at least one fluorescence digital image of the sample; and computing means for processing images for counting thrombocytes automatically present in the or each fluorescence image. |
申请公布号 |
EP2246691(A1) |
申请公布日期 |
2010.11.03 |
申请号 |
EP20100290220 |
申请日期 |
2010.04.27 |
申请人 |
COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIESALTERNATIVES |
发明人 |
FAIVRE, MAGALIE;CAILLAT, PATRICE;CUBIZOLLES, MYRIAM-LAURE;PEPONNET, CHRISTINE;VAUCHIER, CLAUDE |
分类号 |
G01N15/14;G01N33/49;G01N33/86 |
主分类号 |
G01N15/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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