METHOD FOR PREPARING HUMAN T CELL POPULATION HAVING BOTH CYTOTOXIC AND IMMUNOSUPPRESSIVE ACTIVITIES

摘要

Disclosed is a method for efficiently preparing a human T cell population which has both cytotoxic and immunosuppressive activities. Specifically disclosed is a method for preparing a human T cell population, which has both cytotoxic and immunosuppressive activities, comprising the following steps (1) to (4): (1) a step for fractionating mononuclear cells collected from human umbilical cord blood into CD14-positive cells and CD14-negative cells, and further fractionating the CD14-negative cells into CD2-positive/CD14-negative cells and CD2-negative/CD14-negative cells; (2) a step for co-culturing the CD2-positive/CD14-negative cells obtained in step (1) with stromal cells to generate blast cells; (3) a step for adding the blast cells obtained in step (2) to the co-culture of the CD14-positive cells obtained in step (1) with stromal cells to thereby allow the blast cells to proliferate; and (4) a step for allowing the blast cells obtained in step (3) to further proliferate by co-culturing with stromal cells in the presence of interleukin-2 (IL-2) to give a human T cell population which has both cytotoxic and immunosuppressive activities.