发明名称 |
SONDE ZUM SEQUENZIEREN VON NUKLEINSÄURE UND VERWENDUNGSVERFAHREN |
摘要 |
A nanoprobe for sequencing of nucleic acid molecules is provided, as well as methods for using the nanoprobe. In particular examples, the probe includes a polymerizing agent and one or more molecular linkers that carry a chemical moiety capable of reversibly binding to the template strand of a nucleic acid molecule, without being detached from the linker, by specifically binding with a complementary nucleotide in the target nucleic acid molecule. The reversible binding of the chemical moiety on the linker with a complementary nucleotide in the target nucleic acid molecule is indicated by emission of a characteristic signal that indicates pairing of the chemical moiety on the linker with its complementary nucleotide. An example of such a chemical moiety is a nonhydrolyzable nucleotide analog. In particular examples, the polymerizing agent and the chemical moiety are associated with a tag, such as a donor fluorophore and acceptor fluorophore characteristic of the particular type of chemical moiety. |
申请公布号 |
DE602006016984(D1) |
申请公布日期 |
2010.10.28 |
申请号 |
DE20066016984T |
申请日期 |
2006.12.12 |
申请人 |
THE GOVERNMENT OF THE UNITED STATES OF AMERICA ASREPRESENTED BY THE SECRETARY OF THE DEPARTMENT OFHEALTH AND HUMAN SERVICES, ROCKVILLE |
发明人 |
SCHNEIDER, THOMAS D.;LYAKHOV, ILYA G.;NEEDLE, DANIELLE |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|