| 摘要 |
FIELD: medicine. ^ SUBSTANCE: vector based on genome of potato X virus includes functional in plant cell promoter, 5'- untranslated region of genome of potato X virus, gen of RNA-dependent RNA-polymerase of potato X virus, promoter of subgenome RNA of potato X virus, sequence of 5'- untranslated region of RNA 4 of lucerne mosaic virus, 3'- untranslated region of genome of potato X potato virus and functional in plant cell transcription terminator. ^ EFFECT: introduction of nucleotide sequence, corresponding to 5'- untranslated region of RNA 4 of lucerne mosaic virus immediately before beginning of gen, which codes target protein, allows to increase target protein production level in 3-4 times by increasing virus RNA translation efficiency. ^ 1 dwg, 1 tbl, 4 ex |
