CDSE/ZNS QUANTUM DOT, METHOD FOR FABRICATING THE SAME AND METHOD FOR MEANSURING CONCENTRATION OF PLATELET DERIVED GROWTH FACTOR USING THEREOF

摘要

PURPOSE: A cadmium selenide/zinc sulfide quantum dot and a method for measuring concentration of platelet-derived growth factor using the same are provided to simplify the method and to enhance measurement efficiency. CONSTITUTION: A cadmium selenide/zinc sulfide quantum dot comprises platelet growth factor(PDGF) aptamer fixed on the surface. The quantum dot causes FRET(Fluorescence Resonance Energy Transfer) phenomenon by black hole quencher(BHQ). A method for measuring the concentration of platelet-derived growth factor comprises: a step of dispersing platelet-derived growth factor aptamer-fixed cadmium selenide/zinc sulfide quantum dot in a buffer solution to form a first solution; a step of fixing BHA on the surface of oligonucleotide; a step of reacting BHQ-fixed oligonucleotide in the first solution to obtain a second solution; and a step of adding platelet-derived growth factor to the second solution and measuring quantum dot intensity.