发明名称 |
SYSTEM AND METHOD FOR DETECTION OF HLA VARIANTS |
摘要 |
<p>A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci..</p> |
申请公布号 |
WO2010118865(A1) |
申请公布日期 |
2010.10.21 |
申请号 |
WO2010EP02307 |
申请日期 |
2010.04.15 |
申请人 |
ROCHE DIAGNOSTICS GMBH;F. HOFFMANN-LA ROCHE AG |
发明人 |
BENTLEY, GORDON;ERLICH, HENRY, A.;HIGUCHI, RUSSELL, GENE;HOLCOMB, CHERIE |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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