| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a post-column fluorimetric determination-boric acid complex anion exchange method, capable of analyzing mannose-6-phosphate. <P>SOLUTION: The disclosed method is a method for separation analysis of reducing sugars using column chromatography, comprising loading a sample onto an anion exchange column, cleaning the column by allowing to flow a sufficient volume of a first mobile phase consisting of an aqueous solution of boric acid containing a water-soluble inorganic salt through the column concerned; supplying a second mobile phase with an elevated concentration of the salt concerned to elute the reducing sugars; adding a basic amino acid to the eluate, heating; and containing to measure continuously and to record the intensity of fluorescent light emitted under irradiation with an exciting light. <P>COPYRIGHT: (C)2011,JPO&INPIT</p> |
