| 摘要 |
The present invention relates to a method for production of a synchronized adherently growing cell line, wherein a culture bottle with culture medium containing adherent cell line is subjected to a vibration under standard cultivation conditions in an incubator, and after finishing the vibrational treatment, the washed- out cell fraction suspension is removed and transferred to a new culture bottle or taken for analysis. The vibration causes the washout of the poorly adhering mitotic cells to the culture medium and initiates inhibition of their proliferation. The invention further relates to a device for carrying out said method, containing a movable platform (1), flexibly mounted on a stationary support structure (3), said movable platform (1) having attachment means (10) and being connected to a vibrator (5), said vibrator being connected by a cable (6) to a vibration regulator (7) embedded in a box (8), said box (8) holding an energy supply (9). |
