| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of determining anthocyanin by using an organic solvent in place of acetonitrile as a moving phase, in analysis of the anthocyanin by HPLC. SOLUTION: In the method wherein the anthocyanin included in an analysis sample is determined by being separated by high-performance liquid chromatography (HPLC), the moving phase is ethanol and/or water. In the method, for example, a material having a filler (ODS) formed by chemically bonding an octadecyl group to a silica gel carrier is used a column for use in HPLC, and a detection wavelength of the anthocyanin is set at 520 nm, and the anthocyanin included in a sample such as delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside, or cyanidin-3-O-rutinoside can be quantitatively analyzed simply. COPYRIGHT: (C)2011,JPO&INPIT |
