METHOD OF PRODUCING SINGLE-STRANDED DNA

摘要

The invention relates to a method for producing single-stranded DNA and to a kit for use in said method. The method comprises a first PCR stage comprising repeating denaturation, annealing and extension steps, and a second PCR stage comprising denaturation and combined annealing and extension steps. In the method, at least one first and at least one second primer having partly overlapping annealing temperature areas are used.