METHOD FOR CULTURING THE NITRILE HYDRATASE-PRODUCING STRAIN RODOCOCCUS RHODOCHROUS M33
摘要
Method for culturing the nitrile hydratase (I)-producing strain Rhodococcus rhodochrousM33 (DSM 14230) using a medium based on 12-60 mM phosphate buffer that (a) satisfies the cells' requirement for phosphorous and (b) maintains pH 5.5-9 during culture. An independent claim is also included for a medium for the process comprising, per l, 7.9 g sodium dihydrogenphosphate dodecahydrate; 1.8 g potassium dihydrogenphosphate; 0.02-0.04 g cobalt(II) chloride hexahydrate; 1 g magnesium sulfate heptahydrate; 0-10 g maize extract; 20-90 g glucose and 4-20 g urea.
申请公布号
EP1737946(B1)
申请公布日期
2010.10.20
申请号
EP20040725918
申请日期
2004.04.06
申请人
ASHLAND LICENSING AND INTELLECTUAL PROPERTY LLC
发明人
LORENZ, JOSEF;WORONIN, S., P.;KOSOLIN, S., W.;SINGIRZEV, I., N.;DEBABOV, V., G.;YANENKO, A., S.