发明名称 HOMOGENEOUS ENZYMATIC ASSAY FOR VITAMIN B6 AND IMPROVEMENTS IN H2S DETECTION
摘要 Enzymatic methods to determine the concentration of pyridoxal 5'-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme for homocysteinase and related enzymes and measuring the products of the reaction preferably spectrophotometrically. The invention also includes improvements in sensitivity of assays for measuring hydrogen sulfide production by measuring fluorescence as opposed to absorbance of the oxidized product of H2S with N, N-dialkyl p-phenylene diamine.
申请公布号 CA2361077(C) 申请公布日期 2010.10.19
申请号 CA20002361077 申请日期 2000.02.01
申请人 ANTICANCER, INC. 发明人 XU, MINGXU;HAN, QINGHONG;TAN, YUYING
分类号 C12Q1/527;G01N31/00;C12N9/88;C12Q1/00;C12Q1/37;C12Q1/48;C12Q1/52;G01N21/78;G01N33/82 主分类号 C12Q1/527
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